BBa_K731500

BBa_K731500 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K731500
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Francesco Guzzonato
Date created: 2012-08-21 11:00:00
Date modified: 2015-05-08 01:13:06

[LacI + LacIq promoter] reverse + [tac promoter + lac operator] forward



    • Details

    Types
    DnaRegion

    Roles
    promoter

    Regulatory

    Sequences BBa_K731500_sequence (Version 1)

    Description

    This is E.coli lactose-inducibile promoter.

    lac repressor (encoded from lacI gene) is the regulator protein: in absence of lactose the protein binds to lac operator, preventing RNA-polymerase binding and transcription.
    Low, constitutive levels of transcription can be seen anyhow.

    Source

    pET21b

    Sequence Annotation Location Component / Role(s)
    stop
    LacI
    start
    rbs
    LacIq promoter
    BBa_K731300
    lacO operator
    Ptac promoter
    		1,3
    1,1084
    1081,1084
    1085,1092
    1093,1153
    1,1153
    1194,1219
    1162,1188
    		feature/stop stop_codon
    CDS feature/cds
    start_codon feature/start
    feature/rbs ribosome_entry_site
    promoter feature/promoter
    feature/BioBrick engineered_region
    operator feature/operator
    feature/promoter promoter

    igem#experience
    Works
     
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K731500/1
     

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