BBa_K731600

BBa_K731600 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K731600

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Andrea Tassinari, Daniele Rossetto, Giacomo Giacomelli

Date created: 2012-08-22 11:00:00

Date modified: 2015-05-08 01:13:06

Cysteine Desulfhydrase (CysDes)

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K731600_sequence (Version 1)

Description

This part encode for a cysteine desulfhydrase from Treponema denticola, which is known to be a strong enzyme that produces H2S, ammonia and pyruvate starting from cysteine. It is often indicated also as Hly.

Notes

The nucleotide sequence of CysDes was optimized by Genscript, thus it does not correspond to the natural sequence found in Treponema denticola.
The sequence of the lac operator corresponds to the sequence found in pET21b.

Source

CysDes nucleotide sequence was synthesized by Genscript and subcloned into psB1C3 to create part BBa_K731600.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Ptac promoter	1,30	feature/promoter, promoter
LacO operator	35,59	operator, feature/operator
rbs	89,94	feature/rbs, ribosome_entry_site
CysDes	103,1305	feature/cds, CDS
start	103,105	start_codon, feature/start
stop	1300,1305	stop_codon, feature/stop

Other Properties

• igem#experience: Works
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K731600/1

Member of these Collections

• iGEM Parts Registry