Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Giacomo Giacomelli, Anna Depetris
Date created: 2012-08-14 11:00:00
Date modified: 2015-05-08 01:13:06
Platform for terminators analysis under the control of tac promoter
| Types | DnaRegion |
| Roles | plasmid_vector Plasmid_Backbone |
| Sequences | BBa_K731710_sequence (Version 1) |
Description
This is the twin construct of BBa_K731700, with the same two subsequent fluorescent proteins, with T7 promoter replaced with Ptac. This construct allows to test possible differences in terminator efficiency due to different promoter and thus polymerase.We tested both constructs in the E.Coli strain BL21pLysS, that has both polymerases.
Notes
The presence of the promoter cannot be tested with screening digestion, as it has more or less the same T7 promoter`s length. Anyway the screening in amplification strains is much more easy: the right colonies will be intensely red.Source
The Ptac promoter has been inserted with a PCR, the sequence of the promoter has been taken from literature,while the original plamid is pET21b
| Sequence Annotation | Location | Component / Role(s) |
| A206K VENUS T7 TERMINATOR ampicillin resistance gene lacI Ptac lacO mCHERRY | 36,752 843,890 1711,2370 4561,5653 6033,6061 6065,6087 6134,6844 | feature/protein CDS sequence_feature feature/misc feature/protein CDS feature/protein CDS feature/promoter promoter operator feature/operator CDS feature/protein |