BBa_K736000

BBa_K736000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K736000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Lethbridge iGEM High School Team

Date created: 2012-06-15 11:00:00

Date modified: 2015-05-08 01:13:07

Constitutive Promoter (J23100) with MLC (glucose-regulated) binding site

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K736000_sequence (Version 1)

Description

This part is designed to regulate transcription of downstream genes according to glucose concentration.

In the absence of glucose in Escherichia coli cells, MLC is bound to DNA; this prevents transcription. When glucose enters the cell, a phosphoryl group from an endogenous protein (EIIBC-gluc) is transferred to the glucose. When EIIBC-gluc is unphosphorylated, it recruits the MLC protein; that is, the MLC becomes unbound from the DNA, which allows transcription to occur.

Notes

The EIIBC-gluc is responsible for proliferating the glucose signal to allow for transcription to occur.

Source

PCR was done using DNA primers designed for J23100, adding the MLC binding sequence to the 3' end. A modified VF2 was used for the 5' antisense.

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K736000/1

Member of these Collections

• iGEM Parts Registry