BBa_K736001

BBa_K736001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K736001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Lethbridge iGEM High School Team

Date created: 2012-06-15 11:00:00

Date modified: 2015-05-08 01:13:07

TAT signal sequence fused to red fluorescent protein (E1010)

Details

• Types: DnaRegion
• Roles: Reporter; engineered_region
• Sequences: BBa_K736001_sequence (Version 1)

Description

The twin argenine tag (TAT) sequence targets proteins to the membrane for secretion. This signal sequence contains the conserved T-R-R-X-F-L-K sequence motif at the end terminus.

The red fluorescent protein is used as a proof of principle to confirm that the TAT signal sequence is capable of sequestering the protein outside of the cell.

Notes

Red fluorescent protein is an easily detectable reporter, allowing for easy proof of principle.

Source

PCR was done using DNA primers designed for E1010, adding the TAT sequence to the 5' end. A VR primer was used for the 3' antisense.

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K736001/1

Member of these Collections

• iGEM Parts Registry