Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tianhe Wang
Date created: 2012-09-17 11:00:00
Date modified: 2015-05-08 01:13:07
gvpC-GFP fusion protein UH
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K737014_sequence (Version 1) |
Description
This part composed of fusion gene between gvpC-20 and GFP mut 3.1 by using rigidity hinge which Length can achieve 41 ?? and Fad-Fad Angle is 123 ??a The fusion protein is used to detect the expression of gvpC within the cell volume.Notes
New Fusion Protein Systems Designed to Give Soluble Expression in Escherichia coli Gregory D. Davis,* Claude Elisee, Denton M. Newham, Roger G. Harri Received 26 January 1999; accepted 16 May 1999Source
Yes,GVP comes from Planktothrix rubescens strain BC-Pla 9402.| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23106 conserved BBa_B0034 BBa_J23106 BBa_B0034 conserved BBa_J23106 BBa_B0034 conserved BBa_K737014 BBa_B0015 BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop BBa_B0015 BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop BBa_B0015 | 1,35 48,51 44,55 64,98 107,118 111,114 127,161 170,181 174,177 190,1594 1603,1731 1603,1682 1614,1657 1691,1731 1698,1717 1718,1731 1724,1724 1740,1868 1740,1819 1751,1794 1828,1868 1835,1854 1855,1868 1861,1861 1877,1956 1888,1931 1965,2005 1972,1991 1992,2005 1998,1998 1877,2005 | engineered_region feature/BioBrick feature/conserved conserved_region engineered_region feature/BioBrick feature/BioBrick engineered_region feature/BioBrick engineered_region feature/conserved conserved_region feature/BioBrick engineered_region feature/BioBrick engineered_region feature/conserved conserved_region feature/BioBrick engineered_region feature/BioBrick engineered_region feature/BioBrick engineered_region feature/stem_loop stem_loop engineered_region feature/BioBrick feature/stem_loop stem_loop polyA_site feature/polya stop_codon feature/stop feature/BioBrick engineered_region engineered_region feature/BioBrick stem_loop feature/stem_loop engineered_region feature/BioBrick feature/stem_loop stem_loop feature/polya polyA_site stop_codon feature/stop engineered_region feature/BioBrick stem_loop feature/stem_loop engineered_region feature/BioBrick stem_loop feature/stem_loop polyA_site feature/polya feature/stop stop_codon feature/BioBrick engineered_region |