Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chunyan Zhang
Date created: 2012-09-17 11:00:00
Date modified: 2015-05-08 01:13:07
J23106+B0034+gvpC-20psi+B0015
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K737018_sequence (Version 1) |
Description
This part conteins a constitutive promoter J23106, a strong RBS B0034, a coding sequence gvpC-20psi and a double-terminator B0015. This part was examined by SDS-PAGE. We transform this part to Escherichia coli Top10 strain. After culturing in Luria-Bertani broth (with Chloramphenicol) for 24 hours, the E. coli cells was centrifuged at the speed of 8000 rpm. Then we resuspent the cell in 50ul PBS (0.5mol/L) and add 50ul 2XLoading buffer in the EP tube. After boiling in burning water for 5min, the cells was centrifuged at 8000 rpm. Then add 20ul supernate to sample hole and SDS-Paged it at 80V for spacer gel and 120V for separation gel. Finally, we got the gel image and analysed it. At last, we located the protein at 20kDa, which means the protein gvpA was successfully expressed in E. coli.Notes
This part was examined by SDS-PAGE. We transform this part to Escherichia coli Top10 strain. After culturing in Luria-Bertani broth (with Chloramphenicol) for 24 hours, the E. coli cells was centrifuged at the speed of 8000 rpm. Then we resuspent the cell in 50ul PBS (0.5mol/L) and add 50ul 2XLoading buffer in the EP tube. After boiling in burning water for 5min, the cells was centrifuged at 8000 rpm. Then add 20ul supernate to sample hole and SDS-Paged it at 80V for spacer gel and 120V for separation gel. Finally, we got the gel image and analysed it. At last, we located the protein at 20kDa, which means the protein gvpA was successfully expressed in E. coli.Source
Yes.| Access | Instance | Definition |
| public public public public | BBa_J23106 BBa_B0034 BBa_K737017 BBa_B0015 | BBa_J23106 BBa_B0034 BBa_K737017 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23106 BBa_B0034 BBa_K737017 BBa_B0015 | 1,35 44,55 62,565 574,702 | BBa_J23106 BBa_B0034 BBa_K737017 BBa_B0015 |