BBa_K737032

BBa_K737032 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K737032
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Peiran Zhang and Minghao Gong
Date created: 2012-09-17 11:00:00
Date modified: 2015-05-08 01:13:07

Spot42 based small RNA2



Types
DnaRegion

Roles
mature_transcript_region

RNA

Sequences BBa_K737032_sequence (Version 1)

Description

Spot42 is a multitarget small RNA that mediates the discoordinate expression of the E.coli galactose operon and other sugar metabolic pathway,such as galK,nanC,ytfJ,srlA,which are the 5??? leader sequence of the corresponding metabolic enzymes.

Take galK for example,Spot42 causes translation repression by base pairing to RBS in the 5??? leader sequence,then block the recognition of the antiSD sequence on 30S subunit.
It???s a very strong pairing(up to 20bases),but it only causes 2.6 fold repression according to Johannes H. Urban and Jo?? rg Vogel,for it doesn???t result in the degradation of the RNA complex by RNaseE(ssRNA degradation) and RNaseIII(dsRNA degradation),both of them are major enzymes that causes RNA degradation in vivo.Spot42-galK complex degrades in a slow and presently unclear way. The base pairing is initiated by the recognition of seed region,which plays a significant role in the repression efficiency.

Notes

No

Source

No

igem#experience
Works
 
igem#partStatus
Released HQ 2013
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K737032/1