Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wenjie Wu and Peiran Zhang
Date created: 2012-09-18 11:00:00
Date modified: 2015-05-08 01:13:07
constitutive Spot42 generator ligated to lacI generator
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K737043_sequence (Version 1) |
Description
[J23106,P0412]is a LacI generator, which represses tet promotor and can be induced by IPTG.aTc concentration gradient results in different sRNA Spot42 levels in cells when presented R0011, which can be monitored by our galK::GFP generator,where galK is the RBS of GFP and the target for Spot42.
This device was mistaken for aTc inducible sRNA device originally due to some ligation mistake,but we actually found this one is perfect to express full level sRNA to verify sRNA function without induction. When assembly the corresponding part together(constitutive Spot42 generator ligated to TetR generator),the double input platform will work efficiently.
Notes
NoSource
No| Access | Instance | Definition |
| public public public public | BBa_J23106 BBa_P0412 BBa_R0040 BBa_K737058 | BBa_J23106 BBa_P0412 p(tetR) BBa_K737058 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23106 BBa_P0412 BBa_R0040 BBa_K737058 | 1,35 44,1351 1360,1413 1422,1530 | BBa_J23106 BBa_P0412 p(tetR) BBa_K737058 |