Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Huachun Liu
Date created: 2012-08-08 11:00:00
Date modified: 2015-05-08 01:13:08
Theophyline riboswitch regulated RNA Scaffold (clover vision 2)
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K738002_sequence (Version 1) |
Description
Scaffold D0 was constructed from a single RNA module d0, which folded into a duplex with PP7 and MS2 aptamer domains that bind PP7 and MS2 fusion proteins. A riboswitch of theophyline is added in the stucture of D0 to regulate its function.Notes
ttaatacgactcactatagggggggcccgggaaccagcgacgggcccggcagcaccgaggaggactcccacagtcactggggagtcctcgaatacgagctgggcacagaagatatggcttcgtgcccaggaagtgttcgcacttctctcgtattcgccccctagcataaccccttggggcctctaaacgggtcttgaggggtttttttgSource
The secquence was commercially synthesized by??????| Sequence Annotation | Location | Component / Role(s) |
| T7 promoter MS2 aptamer PP7 aptamer T7 terminator Theophylline aptamer | 1,21 76,89 109,134 165,217 31,66 | promoter feature/promoter feature/stem_loop stem_loop feature/stem_loop stem_loop feature/operator operator stem_loop feature/stem_loop |