BBa_K738004

BBa_K738004 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K738004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Huachun Liu

Date created: 2012-08-08 11:00:00

Date modified: 2015-05-08 01:13:08

FA-2X-MS2: Split GFP N-terminal domain fused with MS2 protein

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K738004_sequence (Version 1)

Description

FA is half of split GFP with 2X linker[Gly4Ser]2, fusion expressed with MS2.

Notes

This part should be expressed in e.coli BL21(DE3) because of its T7 promoter and terminator.

Source

Dr. Camille J. Delebecque, the author of Organization of Intracellular Reactions with Rationally Designed RNA Assemblies, offer us FA-2X-MS2 secquence.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
start code	1,3	feature/start, start_codon
FA	1,474	feature/cds, CDS
2X	475,504	feature/protein, CDS
MS2	505,1284	feature/cds, CDS
stop code	1279,1284	feature/stop, stop_codon

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K738004/1

Member of these Collections

• iGEM Parts Registry