RS1

BBa_K743000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K743000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Date created: 2012-09-19 11:00:00
Date modified: 2015-05-08 01:13:09

Synechocystis PCC6803 neutral recombination site.



    • Details

    Types
    DnaRegion

    Roles
    DNA

    sequence_feature

    Sequences BBa_K743000_sequence (Version 1)

    Description

    Any DNA sequence flacked by this part and BBa_K743001 will be incorporated into Synechocistys PCC6803 chromosome through a neutral double homologous recombination.

    Notes

    According to literature, in Synechocystis the recombination sites must be at least 450bp long to allow a rasonably high transformation efficiency, being 700bp the optimum size.

    Source

    PCR amplified from synechocystis PCC6803 chromosome.

    Sequence Annotation Location Component / Role(s)
    RS1 recombination site
    		1,522
    		feature/dna sequence_feature

    igem#experience
    Works
     
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K743000/1
     

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