Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Bryon Silva
Date created: 2012-09-21 11:00:00
Date modified: 2015-05-08 01:13:09
OmpA + HIV protease cleavage site secretion system
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K743011_sequence (Version 1) |
Description
This device is designed for the further incorporation of any protein coding sequence downstream the HIV cleavage site.Upon addition of arabinose to the medium, the inducible Pbad promoter drives the expression of outer membrane protein A (ompA) fused to a extracellular linker followed by a cleavage site recognizable by HIV protease.
If any polypeptide sequence is fused to the 3?? end of the HIV cleavage site DNA sequence, it will protrude from the outer membrane where it will be cleaved from the ompA domain by HIV protease.
By this means, this device works as a arabinose -> protein secretion system.
By this way
Source
All parts were obtained form exciting bricks.| Access | Instance | Definition |
| public public public public | BBa_I0500 BBa_K103006 BBa_I712015 BBa_B0010 | pBad/araC OmpA BBa_I712015 BBa_B0010 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I0500 BBa_K103006 BBa_I712015 BBa_B0010 | 1,1210 1211,1674 1675,1716 1717,1796 | pBad/araC OmpA BBa_I712015 BBa_B0010 |