Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Simon Alamos
Date created: 2012-09-21 11:00:00
Date modified: 2015-05-08 01:13:09
after dusk -> LuxAB luciferase (Photorabdus luminiscens)
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K743014_sequence (Version 1) |
Description
This part is designed to express the LuxA and LuxB genes from Photorabdus luminiscens in a circadian manner, peaking a 2 hours after subjective dusk (or hour 14 of circadian time if you prefer).When transfromed into Syenchocystis PCC6803, the endogenous circadian transcriptional mechanism controls the activity of the Pta promoter making the heterodimeric LuxAB luciferase to be expressed at the specified time.
This permits for the emition of light by the luciferase reaction to be restricted to this time of the day (dependent on the addition or endogenous production of substrates)
Source
LuxAB coding sequence was amplified from http://partsregistry.org/Part:BBa_K216008.Pta promoter is from BBa_K743003
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K743003 Pta promoter putative rbs (not known) LuxA rbs LuxB stop codons BBa_K216008 | 1,150 120,150 151,1230 1231,1247 1248,2228 2229,2234 151,2234 | feature/promoter promoter feature/rbs ribosome_entry_site feature/protein CDS ribosome_entry_site feature/rbs feature/protein CDS feature/tag tag engineered_region feature/BioBrick |