Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lei Qiao
Date created: 2012-09-14 11:00:00
Date modified: 2015-05-08 01:13:12
AgrC protein coding sequence. AgrC is main component of S.aureus agr quorum sensing system.
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K748001_sequence (Version 1) |
Description
AgrC is a transmembrane protein, which is the sensor molecule of a typical two-component signal system in S.aureus. AgrC possesses several key amino acid motifs typical of histidine protein kinase sensor. The AgrC sensor kinase can specifically binds to corresponding AIP, which secreted only from specific S.aureus, and the composite of AgrC and AIP then leads to phosphorylation of AgrA. AgrA in its phosphorylated sate activates transcription from both P2 and P3, leading to the production of GFP and 3OC6HSL. Thus we can detect the presence of S.aureus expediently by observing the expression of GFP.Notes
we employed site-specific mutagenesis(AgrC-I A101T) to replace a amino acid residue in AgrC-I so that the mutated AgrC can bound to both AIP-I and AIP-IV.Source
Staphylococcus aureus genomic sequence| Sequence Annotation | Location | Component / Role(s) |
| AgrC-I A101T | 301,303 | sequence_alteration feature/mutation |