Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Rachel Engeler
Date created: 2012-08-28 11:00:00
Date modified: 2015-05-08 01:13:12
Yeast FIG1 promoter
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K753000_sequence (Version 1) |
Description
Promoter of the FIG1 gene in yeast. The transcription factor Ste12, activated in the yeast pheromone pathway, binds to the FIG1 promoter and initiate the expression of the FIG1 gene.Five pheromone response elements (PRE, consensus sequence ATGAAACA) have been identifi?ed in the 5'-upstream regulatory sequence of the FIG1 gene. Phosphorylated Ste12 binds to them and thereby activates transcription. The PREs in the FIG1 promoter di?ffer slightly from the PRE consensus sequence ATGAAACA:
Sequence Location
TGAAACG -405
TGAAACC* -217
TGACACA* -206
TGAAACG -173
TGAAATA -133
Sites marked with an asterisk occur on the opposite strand
The strictly regulated transcriptional feature of the FIG1 promoter can be useful when investigating the pheromone pathway. The promoter can be used to express reporter genes in response to activation of the pheromone pathway.
Reference: Erdman S, Lin L, Malczynski M, Snyder M. Pheromone-regulated Genes Required for Yeast
Mating Diff?erentiation. The Journal of cell biology. 1998;140(3):461-483.
Notes
The length of the promoter was chosen to 450 bp as recommended by Kondo group of Kobe University of Japan.Togawa S, Ishii J, Ishikura A, Tanaka T, Ogino C, Kondo A. Importance of asparagine residues at positions 13 and 26 on the amino-terminal domain of human somatostatin receptor subtype-5 in signalling. J Biochem. 2010 Jun;147(6):867-73