| Types | DnaRegion
|
| Roles | Coding
CDS
|
| Sequences | BBa_K759014_sequence (Version 1)
|
Description
This Ag43 protein coding site was derived from E. coli K-12 strain and it's codons are optimized to remove 6 PstI recognition sites that it originally had, and for being adapted to E. coli K-12 strain codon usage. E. coli cell-to-cell auto-aggregation would be improved (particularly about expression of this protein) by this optimization.
Notes
This coding site contains BsrGI, NcoI and SacI restriction enzyme recognition sites. Because of difficulty of synthesizing whole sequence at a time, we planned to assemble this part by shortly synthesized parts with these enzymes.
Source
This coding site is referred to BBa_K346007 which was derived from E. coli K-12 strain.
