BBa_K763006

BBa_K763006 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K763006
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Luisa Mar??a Mart??nez S??nchez
Date created: 2012-09-16 11:00:00
Date modified: 2015-05-08 01:13:13

pTRR + Gene encoding ZsGreen1



Types
DnaRegion

Roles
engineered_region

Reporter

Sequences BBa_K763006_sequence (Version 1)

Description

The expression of the thioredoxin reductase (TRR) promoter are regulated by the transcription factors who works together YAP1 and SKN7 in response to oxidative stress. YAP1 transcription factor is regulated by subcellular localization, under normal conditions a export receptor (Crm1) escorts YAP1 from the nucleus to the cytoplasm where is stored. In the presence of hydrogen peroxide, another protein (Hyr1) catalyces the formation of an intramolecular disulfide bond in YAP1. This conformational change allows YAP1 to accumulate in the nucleus and binds to the TRR promoter by a bZIP DNA-binding domain. In the other side, Skn7 transcriptional factor is constitutively nuclear. In the presence of hydrogen peroxide it is able to associate with TRR promoter. Some studies suggest that the formation of the skn7+ yap1+ DNA complex requires more than both transcription factor binding to the promoter in close proximity, and the fact that both proteins interact directly, in conclution the two transcription factors requires only one biding site in the promoter (Association of the???). In the presence of the two transcriptional factor binding in to the TRR promoter the transcription of the green fluorescent protein starts.

Notes

No design considerations needed.

Source

The sequence of the promoter comes from yeast Sacharomyces cerevisiae genome, whereas the sequence encoding the ZsGreen1 fluorescent protein is not natural, since it was optimized for codon usage and maximum fluorescence production.

igem#experience
Works
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K763006/1