BBa_K772004

BBa_K772004 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K772004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Mustafa Elitok

Date created: 2012-09-24 11:00:00

Date modified: 2015-05-08 01:13:15

AHL regulated PoPS generator: Las receiver device

Details

• Types: DnaRegion
• Roles: engineered_region; Generator
• Sequences: BBa_K772004_sequence (Version 1)

Description

Quorum sensing is the mechanism of communication between bacteria via synthesizing and detecting chemical compounds in the media. With these mediators, the promoters and their yield can be tuned or regulated.
This part represents an alternative quorum sensing receiver device, which is able to generate a PoPS response in the presence of 6HSLO3 produced by LasI, instead of BBa_F2620. This will allow iGEMers to establish communications between different bacteria colonies and it makes simple to generate easy regulated complex systems.
This part is assembled with Las promoter system to improve the current quorum sensing devices and parts which commonly base on Lux promoter system.

Notes

Note that pLas has no RBS afterwards, the assembling methods must be selected after this consideration.

Source

pLas and LasR sequences are gathered from Registry.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
pTetR	1,54	promoter, feature/promoter
RBS	55,75	feature/rbs, ribosome_entry_site
LasR	76,824	feature/BioBrick, engineered_region
Double Terminator	825,954	feature/stop, stop_codon
pLas	955,1122	promoter, feature/promoter

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/myers
• synbiohub#ownedBy: user/james
• synbiohub#topLevel: BBa_K772004/1

Member of these Collections

• //cds/transcriptionalregulator
• //chassis/prokaryote/ecoli
• //classic/regulatory/other
• //classic/signalling/receiver
• //function/regulation/transcriptional
• //regulator
• iGEM Parts Registry