BBa_K772005

BBa_K772005 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K772005

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Mustafa Elitok

Date created: 2012-09-24 11:00:00

Date modified: 2015-05-08 01:13:15

AHL generator: LasI

Details

• Types: DnaRegion
• Roles: engineered_region; Generator
• Sequences: BBa_K772005_sequence (Version 1)

Description

Quorum sensing is the mechanism of communication between bacteria via synthesizing and detecting chemical compounds in the media. With these mediators, the promoters and their yield can be tuned or regulated.
This part represents an alternative quorum sensing sender device which is designed to induce PoPS production in the parts including pLas such as BBa_K772004. LasI is an enzyme generator gene sequences which synthesizes 6HSLO3 (AHL) in order to induce a PoPS response in the pLas promoter. pLas promoter works in the presence of both 6HSLO3 and LasR protein.

Notes

Note that there is an RBS sequence just before LasI; in order to be easily used with quorum sensing receiver devices such as BBa_F2620 or BBa_K772004.

Source

LasI sequence is gathered from Registry.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
LacI	1,1132	engineered_region, feature/BioBrick
pLac	1133,1331	feature/promoter, promoter
RBS	1332,1352	ribosome_entry_site, feature/rbs
Terminator	1969,2098	feature/stop, stop_codon
LasI	1353,1968	feature/protein, CDS

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K772005/1

Member of these Collections

• //cds/transcriptionalregulator
• //chassis/prokaryote/ecoli
• //classic/regulatory/other
• //classic/signalling/sender
• //function/regulation/transcriptional
• //regulator
• iGEM Parts Registry