Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Mustafa Elitok
Date created: 2012-09-24 11:00:00
Date modified: 2015-05-08 01:13:15
GFP - LacZ anchor-inducer protein complex
| Types | DnaRegion |
| Roles | engineered_region Translational_Unit |
| Sequences | BBa_K772006_sequence (Version 1) |
Description
This part contains a green fluorescent protein (GFP) as a non-reactive chemical compound (anchor) in order to neutralize the active reactive fixed to it, a reporter/inducer device or protein (enzyme etc.) and a TEV protease cleavage site between them to assemble these two proteins. BBa_772001 and BBa_K772002 are assembled to be used as the trigger of detection in the whole system which ends with the intracellular transfection (or activation) of TEV protease. After the activation, it is planned that TEV protease will cleave this anchor-inducer protein complex to release free the inducer/reporter.In conclusion, the inducer would activate a transcription pathway on the targeted inducible gene or the reporter would indicate that there is a change in the whole system.
Notes
Note that there is an RBS sequence just before the protein sequence in order to eliminate the need of addition of it.Source
Cleavage site, LacZ and GFP sequences are gathered from Registry.| Sequence Annotation | Location | Component / Role(s) |
| RBS GFP TEV cleavage site LacZ Double Terminator Anchor - Reporter | 1,20 21,741 742,831 832,1075 1076,1207 21,1075 | feature/rbs ribosome_entry_site feature/BioBrick engineered_region CDS feature/cds feature/BioBrick engineered_region feature/stop stop_codon feature/protein CDS |