Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Mustafa Elitok
Date created: 2012-09-25 11:00:00
Date modified: 2015-05-08 01:13:15
GFP - LasR anchor-inducer protein complex
| Types | DnaRegion |
| Roles | Translational_Unit engineered_region |
| Sequences | BBa_K772007_sequence (Version 1) |
Description
This part contains a green fluorescent protein (GFP) as a non-reactive chemical compound (anchor) in order to neutralize the active reactive fixed to it, an inducer regulatory protein (LasR is responsible to induce pLas promoter in the presence of 6HSLO3 which is a chemical compound sythesized by an enzyme that LasI gene codes.) and a TEV protease cleavage site between them to assemble these two proteins. BBa_772001 and BBa_K772002 are assembled to be used as the trigger of detection in the whole system which ends with the intracellular transfection (or activation) of TEV protease. After the activation, it is planned that TEV protease will cleave this anchor-inducer protein complex to release free the reporter. In conclusion, in this system, the inducer would activate a transcription pathway on the targeted inducible gene or the reporter would indicate that there is a change in the whole system.Notes
Cleavage site, LasR and GFP sequences are gathered from Registry.Source
Note that there is an RBS sequence just before the protein sequence in order to eliminate the need of addition of it.| Sequence Annotation | Location | Component / Role(s) |
| RBS GFP TEV cleavage site LasR Anchor - Inducer Double Terminator | 1,20 21,741 782,871 880,1636 21,1636 1636,1720 | ribosome_entry_site feature/rbs feature/BioBrick engineered_region feature/cds CDS feature/BioBrick engineered_region CDS feature/protein stop_codon feature/stop |