Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: NRP-UEA-Norwich
Date created: 2012-06-24 11:00:00
Date modified: 2015-05-08 01:13:15
Mammalian-Bacterial Promoter: E9-ns2 CArG promoter sequence + BHN + BBaK216005
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K774001_sequence (Version 1) |
Description
A hybrid of the PyeaR promoter (BBaK216005) and the E9-ns2 CArG promoter (Ref1). Two versions will be synthesised, with the promoters altering their position in relation to the 5???-end of the sequence.Notes
To provide additional restriction enzyme sites that may become useful during later cloning steps, BamHI, HindIII and NdeI have been added between the 2 promoters.It is envisaged that any open reading frame (e.g. RFP or GFP) will be cloned ???downstream??? (i.e. at the 3???-end) of these promoter sequences.
Source
PyeaR promoter (BBaK216005) and the E9-ns2 CArG promoter (Ref1)Ref1: Scott, S.D., Joiner, M.C. & Marples, B., 2002. Optimizing radiation-responsive gene promoters for radiogenetic cancer therapy. Gene therapy, 9(20), p.1396-402. Available at: http://www.ncbi.nlm.nih.gov/pubmed/12365005 [Accessed June 24, 2012].
| Sequence Annotation | Location | Component / Role(s) |
| E9-ns2 CArG promoter BHN BBaK216005 | 1,90 91,109 110,208 | promoter feature/promoter feature/misc sequence_feature feature/promoter promoter |