Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sietske Grijseels
Date created: 2012-08-30 11:00:00
Date modified: 2015-05-08 01:13:15
RI7-GPR109A Niacin chimeric GPCR
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K775000_sequence (Version 1) |
Description
BBa_K775000 encodes a chimeric niacin-sensing G-protein coupled receptor which can be functionally expressed in S. cerevisiae. This chimeric receptor is composed of the ligand-binding domain of the mammalian GPR109A, flanked by the N- and C- terminals of the rat OR RI7.Notes
Promoter: GPDTerminator: CYC1.
Source
Homo sapiens NP_808219.1Rat
| Sequence Annotation | Location | Component / Role(s) |
| GPD promoter BamHI restriction site Kozak sequence Startcodon FLAGtag N terminus RI7 Stopcodon NdeI restriction site CYC1 terminator C terminus RI7 Niacin receptor part | 1,112 113,118 119,124 125,127 128,151 152,337 1151,1156 1157,1162 1163,1414 1055,1150 338,1054 | feature/promoter promoter sequence_feature feature/misc ribosome_entry_site feature/rbs feature/start start_codon tag feature/tag sequence_feature feature/misc stop_codon feature/stop feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc feature/protein CDS |