Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Flash Coli
Date created: 2012-09-17 11:00:00
Date modified: 2015-05-08 01:13:16
fliC
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K777109_sequence (Version 1) |
Description
- The fliC gene codes for flagellin. This protein is the subunit that forms the flagellar filament of E. Coli.
Notes
- The fliC gene contained three PstI sites and one SpeI site that had to be removed. These mutations were induced with different primers via overlap-PCR.
- flhDC was amplified from genomic DNA of E. coli DH10B via PCR using the following primers:
- Fwd: ACTGAATTCGCGGCCGCTTCTAGATGCATACCTCCGAGTTGCTG
- Rev: TCCTGCAGCGGCCGCTACTAGTTACTGCCCGGGATGGCGGTTGACATAAGCcGCAGGCAAAGCTGCCAACAG
Source
- The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1).
| Sequence Annotation | Location | Component / Role(s) |
| FliC coding region ATG start codon TAA stop codon SpeI site removed PstI site removed PstI site removed PstI site removed | 1,1497 1,3 1495,1497 552,552 876,876 921,921 1488,1488 | CDS feature/cds feature/start start_codon stop_codon feature/stop point_mutation feature/s_mutation point_mutation feature/s_mutation point_mutation feature/s_mutation feature/s_mutation point_mutation |