BBa_K777118

BBa_K777118 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K777118
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Team Goettingen
Date created: 2012-09-20 11:00:00
Date modified: 2015-05-08 01:13:17

motB under the control of constitutive promoter J23100



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K777118_sequence (Version 1)

Description

This gene codes for MotB, a part of the flagellar motor. MotA and MotB constitute the stator and are required for the flow of ions and torque generation.

Notes

  • motB was amplified from genomic DNA of E. coli DH10B via PCR using the following primers:
    • Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgaagaatcaagcgcatcc-3'
    • Rev: 5'-gtttcttcctgcagcggccgctactagtatcacctcggttcggctgatgg-3'


Source

  • The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1).
  • J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.


igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K777118/1