Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kristjan Eerik Kaseniit
Date created: 2012-09-28 11:00:00
Date modified: 2015-05-08 01:13:18
Long reporter bottom strand MammoBlock
| Types | DnaRegion |
| Roles | sequence_feature Other |
| Sequences | BBa_K779502_sequence (Version 1) |
Description
Long reporter bottom strand with fluorophore to bind to BBa_K779501 and BBa_K779500.Modifications: 3' ROX florophore, all bases modified with 2'O Methyl (ordered as a DNA oligo with Us instead of Ts).
IDT DNA oligo order: mUmGmG mAmUmA mGmUmU mGmGmU mGmGmG mAmGmA mGmGmA mGmUmG mGmGmU mG/3ROX_N/
Notes
Sequence designed to be orthogonal to the HEK293 transcriptome, and to have a high melting temperature.See more details at the MIT iGEM wiki: http://2012.igem.org/Team:MIT
Source
De novo design.| Sequence Annotation | Location | Component / Role(s) |
| Toehold* Domain Sa* | 1,8 9,28 | feature/misc sequence_feature feature/misc sequence_feature |