BBa_K783056

BBa_K783056 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K783056
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Traci Haddock
Date created: 2012-10-01 11:00:00
Date modified: 2015-05-08 01:13:21

Level 0 MoClo Destination Vector with EB



Types
DnaRegion

Roles
Plasmid

plasmid

Description

This is a Level 0 MoClo destination vector with flanking sites E on the 5' side and site B on the 3' side of the alpha fragment of lacZ.

The fusion site letters refer to 4bp fusion sites: A = GGAG; E = GCTT; B = TACT; F = CGCT; C = AATG; G = TGCC; D = AGGT; H = ACTA.

Notes

We had to confirm the lacZ orientation was 5'->3' after cloning.

Source

We amplified the alpha lacZ fragment from pUC19 and used the BioBrick pSB1C3 backbone for our vector backbone.

igem#sampleStatus
No part sequence
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K783056/1