BBa_K783063

BBa_K783063 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K783063

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Traci Haddock

Date created: 2012-10-01 11:00:00

Date modified: 2015-05-08 01:13:21

Level 1 MoClo Destination Vector with EF

Details

• Types: DnaRegion
• Roles: plasmid; Plasmid

Description

This is a Level 1 MoClo destination vector with flanking sites E on the 5' side and site F on the 3' side of the alpha fragment of lacZ.

The fusion site letters refer to 4bp fusion sites: A = GGAG; E = GCTT; B = TACT; F = CGCT; C = AATG; G = TGCC; D = AGGT; H = ACTA.

Notes

We had to confirm the lacZ orientation was 5'->3' after cloning.

Source

We amplified the alpha lacZ fragment from pUC19 and used the BioBrick pSB1C3 backbone for our vector backbone.

Other Properties

• igem#sampleStatus: No part sequence
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K783063/1

Member of these Collections

• iGEM Parts Registry