Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Noa Katz
Date created: 2012-09-12 11:00:00
Date modified: 2015-05-08 01:13:21
pT3_RBS_xyIE reporter gene
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K784004_sequence (Version 1) |
Description
This part's purpose is to testify to the presence of the T3 RNA Polymerase by the xyIE reporter gene from iGEM2006_Edinburgh. It consists of an RNA T3 promoter, an RBS, a reporter gene- xyIE, and a T7 terminator. The assay for xyIE is very simple, refer to the xyIE basic part for instructions.The xyIE+RBS part is between XhoI and XmaI restriction sites, and therefore can be excised by these enzymes.
Notes
No particular considerations were required.Source
The RBS and xyIE genes are from the parts registry, made by iGEM2006_Edinburgh.The promoter and terminator are from:
http://nar.oxfordjournals.org/content/early/2012/06/28/nar.gks597.full
| Access | Instance | Definition |
| public public public | BBa_K784003 BBa_J33204 BBa_K784002 | BBa_K784003 BBa_J33204 BBa_K784002 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K784003 BBa_J33204 BBa_K784002 | 1,23 32,989 998,1044 | BBa_K784003 BBa_J33204 BBa_K784002 |