Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Asaf Blumental
Date created: 2012-09-25 11:00:00
Date modified: 2015-05-08 01:13:22
T7*(T3) RNAP double stop codon
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K784052_sequence (Version 1) |
Description
This part includes T7*(T3) RNA Polymerase gene. This is orthogonal polymerases, based on T7 wt RNAP, that recognizes specifically a suitable promoter- pT3. The T7*(T3) RNA Polymerase was donated us by Chris Voight lab (see source). The T7*(T3) RNA Polymerase is less toxic to host cells, than the T7 wt. In order to achieve reduced levels of toxicity, Chris and his team had added N-terminal degradation tag to the T7 wt polymerase to reduce its concentration in host E.coli cell and controlled the RNAP expression using a weak ribosome-binding site and ATG start codon. The specificity of the T7*(T3) to pT3 was achieved by engineering a DNA binding domain within the RNA polymerase.Notes
No particular considerations.Source
Temme Karsten, et al. 2012. Modular control of multiple pathways using engineered orthogonal T7 polymerases. Nucleic Acids Research (advance access): 1-9.| Sequence Annotation | Location | Component / Role(s) |
| proposed umu tag T7*(T3) RNAP | 1,120 1,2778 | tag feature/tag CDS feature/cds |