Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Zining Hou
Date created: 2012-09-22 11:00:00
Date modified: 2015-05-08 01:13:22
Lux(CDABEG) coding gene PoPS->Light
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K785003_sequence (Version 1) |
Description
This page described the Lux operon from Vibrio fischeri. Lux gene is directly PCRed from the BBa_K325909, the pbad operon is deleted and now this part is available for any expression unit. You can provide light out put by putting this coding device under your promoter.We have successfully put this coding sequence into the PET28a expression system (cell strain is dH5α), and light out put could induced by the IPTG.
To see more information about Lux light generator, please visit http://partsregistry.org/Part:BBa_K325909, and a more complete description can be found on the http://2010.igem.org/Team:Cambridge/Bioluminescence/G28.
Notes
In our project, we have to put the Lux light generator gene under the Ptet operon. So we have to relieve this part from the Pbad operon.Source
We use PCR extract the DNA sequence from BBa_K325909, and clone it into the PSB1C3.| Sequence Annotation | Location | Component / Role(s) |
| putative rbs region luxD luxC putative rbs region putative rbs region luxA putative rbs region luxB putative rbs region luxE putative rbs region luxG | 1,20 1465,2368 21,1450 1454,1458 2406,2411 2419,3483 3509,3514 3522,4502 4565,4571 4616,5713 5705,5709 5717,6427 | feature/rbs ribosome_entry_site CDS feature/cds feature/cds CDS feature/rbs ribosome_entry_site ribosome_entry_site feature/rbs feature/cds CDS feature/rbs ribosome_entry_site feature/cds CDS feature/rbs ribosome_entry_site feature/cds CDS ribosome_entry_site feature/rbs feature/cds CDS |