Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Leung Wai Tak
Date created: 2012-09-20 11:00:00
Date modified: 2015-05-08 01:13:22
Sensory rhodopsin I (SRI) with HtrII & Tar, sensitive to orange light
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K786003_sequence (Version 1) |
Description
Sensory rhodopsin I fusion with HtrII & Tar, which is sensitive to orange light, with sensing spectra covering from 590-650 nm.We used it for sensing blur-green light to achieve phototaxis and switch for downstream gene expression.
Notes
Pfam (version 26.0) was used to predict the domain of fusion protein.Linker was refereed and modified from previous research study of SRI fusion with HtrII.
Source
From genomic sequence of bacterial N. pharaonis (DSM 2160) and E.coli K12.| Sequence Annotation | Location | Component / Role(s) |
| Constitutive promoter BBa_J23100 RBS 1.0 SRI-HtrI-Tar B0015 | 1,41 42,61 68,1992 2029,2158 | feature/promoter promoter feature/rbs ribosome_entry_site sequence_feature feature/misc engineered_region feature/BioBrick |