Types | DnaRegion
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Roles | RNA
mature_transcript_region
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Description
BBa_K788002 can be transcribe into pri-miRNA and then finally processed into mature amiRNA designed to interfere mammal's PGC1-α expression. This gene came originally from Arabidopsis thaliana. Replacement of its natural miRNA segment to a designed one is used to produce artificial miRNAs.
You can redesign this part into generator for other ami-RNA, and make it by overlapping RCR or Gibson Assembly easily. Detail for this method please visit "http://2012.igem.org/Team:Nanjing-China".
Notes
The backbone of this part has been documented a high efficient natural mi-RNA generator in Arabidopsis and other green plant.
Source
From Arabidopsis thaliana's genome.