PolyHb

BBa_K792007 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K792007
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Manuel Gim??nez
Date created: 2012-09-24 11:00:00
Date modified: 2015-05-08 01:13:23

Histidine rich peptide (PolyHb)



Types
DnaRegion

Roles
polypeptide_domain

Protein_Domain

Sequences BBa_K792007_sequence (Version 1)

Description

Stable and soluble Histidine rich peptide.

This part has a HindIII restriction site as extra.

Notes

PolyHb was desing taking into acount the following consideration:
  1. avoided repeating the same residue in tandem to minimize local tRNA depleation
  2. avoided Trp in tandem because of therir low solubility
  3. we included Gly to avoid the formation of stable structures
  4. included acidic and basic amino acids to increase solubility



Sequence obtained by retro-translation. Codon and mRNA-secondary structure optimized for yeast.

Source

Designed by BsAs Team 2012

Sequence Annotation Location Component / Role(s)
HindIII restriction site
stop
cds
1,6
115,120
7,114
sequence_feature feature/misc
stop_codon feature/stop
CDS feature/cds
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K792007/1