Types | DnaRegion
|
Roles | polypeptide_domain
Protein_Domain
|
Sequences | BBa_K792007_sequence (Version 1)
|
Description
Stable and soluble Histidine rich peptide.
This part has a HindIII restriction site as extra.
Notes
PolyHb was desing taking into acount the following consideration:
- avoided repeating the same residue in tandem to minimize local tRNA depleation
- avoided Trp in tandem because of therir low solubility
- we included Gly to avoid the formation of stable structures
- included acidic and basic amino acids to increase solubility
Sequence obtained by retro-translation. Codon and mRNA-secondary structure optimized for yeast.
Source
Designed by BsAs Team 2012