BBa_K798000

BBa_K798000 Version 1

Component

This part has been discontinued.

Source:
http://parts.igem.org/Part:BBa_K798000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Taijie Jin
Date created: 2012-09-23 11:00:00
Date modified: 2015-05-08 01:13:23

450bp overlong telomere repeats of Saccharomyces cerevisiae



Types
DnaRegion

Roles
sequence_feature

Other

Sequences BBa_K798000_sequence (Version 1)

Description

This part is a 450bp overlong telomere repeats of Saccharomy cescerevisiae(its general length is about 300bp). According to some researches before, the overlong telomere in the yeast will decrease by 2-3bp per generation. Thus, we can insert some key regulation sequence (but the sequence has to be very small, <100bp) in the overlong part of the telomere sequence. After recombination into the yeast chromosome, the telomere begins to decrease and about 70 generations later the key sequence will be cut off which can have an effect on the regulation. Obviously, the main advantage of the design is that we can change the regulation procedurally and predictably.

Notes

We inserted a ribozyme sequence shorter than 40bp into the overlong part of the telomere. The ribozyme sequence is able to recognize the target substrate sequence in the 5??? UTR region of the target gene and then down-regulate the gene expression. If the target gene is negative for an essential gene of Yeast, after the ribozyme sequence is damaged with the decrease of the telomere (after about 70 generations), the yeast will be dead. Thus, it can be used to construct an engineering yeast with a fixed life span.

Source

Synthetized based on the character of the yeast's telomere sequence

Sequence Annotation Location Component / Role(s)
Telomere repeats
22,465
feature/dna sequence_feature
igem#experience
None
 
igem#sampleStatus
Discontinued
igem#status
Deleted
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K798000/1