BBa_K798003

BBa_K798003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K798003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Taijie Jin
Date created: 2012-09-23 11:00:00
Date modified: 2015-05-08 01:13:23

Ribozyme sequence



Types
DnaRegion

Roles
DNA

sequence_feature

Sequences BBa_K798003_sequence (Version 1)

Description

This part is a ribozyme sequence whose design is based on the phenomena that structured RNAs embedded in the untranslated regions (UTRs) of messenger RNAs can regulate gene expression (Martick, Horan et al. 2008). It can be inserted with its target substrate sequence into the 5???UTR region of any genes and as a result down-regulate the expression of the gene or even entirely silence the gene. The ribozyme sequence should be after the substrate sequence. Besides, the length of interval sequence between ribozyme and substrate is alterable from 0 to nearly 1000 bp.

Notes

Due to its short length and its down-regulation function for target genes, we insert it into the overlong telomere part as a regulation switch. Once it is damaged with the decrease of telomere, the switch will be off and the expression of the target gene will be normal.

Source

Designed and synthetized based on some ribozyme researches

Sequence Annotation Location Component / Role(s)
Ribozyme
13,41
feature/dna sequence_feature
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K798003/1