Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dennis Hell
Date created: 2012-09-20 11:00:00
Date modified: 2015-05-08 01:13:24
Yeast expression cassette for CaXMT1 and CaMXMT1
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K801076_sequence (Version 1) |
Description
Yeast Expression Cassette for the enzymes 1 and 2 of the caffeine biosynthesis pathwayExpression of CaXMT1 is controlled by the yeast TEF2 promoter and the yeast ADH1 terminator.
Expression of CaMXMT1 is controlled by the yeast TEF1 promoter and the yeast ADH1 terminator.
Notes
-Source
Composite part of BBa_K801010, BBa_K801070, BBa_K801012, BBa_K319003, BBa_K801071 and BBa_K801012| Sequence Annotation | Location | Component / Role(s) |
| TEF2 promoter Cassette 1 + Cassette 2 CaXMT1 ADH1 Terminator TEF1 promoter CaMXMT1 ADH1 Terminator Consensus sequence Strep-Tag Stop codon Consensus sequence Strep-Tag Stop codon Start codon Start codon | 1,648 1,4171 663,1778 1823,2172 2181,2631 2644,3777 3823,4171 657,662 1779,1808 1809,1814 2638,2643 3778,3807 3808,3813 663,665 2644,2646 | promoter feature/promoter engineered_region feature/BioBrick CDS feature/cds feature/misc sequence_feature feature/promoter promoter feature/cds CDS sequence_feature feature/misc feature/misc sequence_feature tag feature/tag stop_codon feature/stop feature/misc sequence_feature tag feature/tag feature/stop stop_codon feature/start start_codon start_codon feature/start |