Types | DnaRegion
|
Roles | Plasmid
plasmid
|
Sequences | BBa_K802003_sequence (Version 1)
|
Description
The shuttle vector is Ampicillin resistant in E. coli and Erythromycin resistant in B. subtilis. It is a low plasmid copy number.
Notes
The SpeI site from the B. subtilis coding region of the pHT304 plasmid was eliminated by filling-in. Afterwards, an iGEM linker containing all 4 of the restriction enzyme sites (EcoRI,XbaI,SpeI,PstI) was cloned into the modified vector.
Source
The shuttle vector is derived from pHT304 (Arantes & Lereclus, 1991)