BBa_K802003

BBa_K802003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K802003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sandu Ioana
Date created: 2012-09-19 11:00:00
Date modified: 2015-05-08 01:13:25

Shuttle vector for E. coli and B. subtilis



Types
DnaRegion

Roles
Plasmid

plasmid

Sequences BBa_K802003_sequence (Version 1)

Description

The shuttle vector is Ampicillin resistant in E. coli and Erythromycin resistant in B. subtilis. It is a low plasmid copy number.

Notes

The SpeI site from the B. subtilis coding region of the pHT304 plasmid was eliminated by filling-in. Afterwards, an iGEM linker containing all 4 of the restriction enzyme sites (EcoRI,XbaI,SpeI,PstI) was cloned into the modified vector.

Source

The shuttle vector is derived from pHT304 (Arantes & Lereclus, 1991)

igem#experience
Works
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K802003/1