BBa_K802004

BBa_K802004 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K802004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sandu Ioana

Date created: 2012-09-19 11:00:00

Date modified: 2015-05-08 01:13:25

Shuttle vector for E. coli and B. subtilis

Details

• Types: DnaRegion
• Roles: plasmid; Plasmid
• Sequences: BBa_K802004_sequence (Version 1)

Description

The shuttle vector is Ampicillin resistant in E. coli and Erythromycin resistant in B. subtilis. It is a high plasmid copy number in B. subtilis.

Notes

The SpeI site from the B. subtilis coding region of the pHT315 plasmid was eliminated by filling-in. Afterwards, an iGEM linker containing all 4 of the restriction enzyme sites (EcoRI,XbaI,SpeI,PstI) was cloned into the modified vector.

Source

The plasmid is derived from the pHT315 vector (Arantes and Lereclus, 1991).

Other Properties

• igem#experience: Works
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K802004/1

Member of these Collections

• iGEM Parts Registry