BBa_K802004

BBa_K802004 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K802004
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sandu Ioana
Date created: 2012-09-19 11:00:00
Date modified: 2015-05-08 01:13:25

Shuttle vector for E. coli and B. subtilis



    • Details

    Types
    DnaRegion

    Roles
    plasmid

    Plasmid

    Sequences BBa_K802004_sequence (Version 1)

    Description

    The shuttle vector is Ampicillin resistant in E. coli and Erythromycin resistant in B. subtilis. It is a high plasmid copy number in B. subtilis.

    Notes

    The SpeI site from the B. subtilis coding region of the pHT315 plasmid was eliminated by filling-in. Afterwards, an iGEM linker containing all 4 of the restriction enzyme sites (EcoRI,XbaI,SpeI,PstI) was cloned into the modified vector.

    Source

    The plasmid is derived from the pHT315 vector (Arantes and Lereclus, 1991).

    igem#experience
    Works
     
    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K802004/1
     

    Attachments

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