Types | DnaRegion
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Roles | sequence_feature
Other
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Sequences | BBa_K802005_sequence (Version 1)
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Description
The linker was cloned into the shuttle vectors BBa_K802004 and BBa_K802003 so that the plasmids would correspond to the iGEM format.
Notes
The cloning sites of the linker are EcoRI and HindIII (there is no need to digest the linker, the two extremities are sticky ends). Between these sites there are the other 3 iGEM enzyme restriction sites: XbaI, SpeI, PstI.
Source
The linker was obtained by the hybridization of two synthesized oligos.