Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Joe Ho
Date created: 2012-10-01 11:00:00
Date modified: 2015-05-08 01:13:25
Constitutive ECFP generator - Arabinose inducible TrpA coding gene
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K804007_sequence (Version 1) |
Description
This part contains a constitutive ECFP generator along with an arabinose inducible (Pbad) Trp A coding gene. Upon induction with arabinose, TrpA is expressed for indole-3-glycerol-phosphate(InGP)binding and catalysis/cleavage of InGP to indole and glyceraldehyde-3-phosphate during an α reaction. This construct can be used to induce growth in Trp- auxotrophs and can be monitored by fluorescence for its population dynamics in co-culture with other auxotrophs.Notes
None available.Source
Strong Constitutive Promoter-ECFP generator- BBa_K804000Pbad inducible Promoter- BBa_I13453
RBS-BBa_B0034
TrpA coding gene- BBa_K804002
Terminator- BBa_B0025
| Access | Instance | Definition |
| public public public public public | BBa_K804000 BBa_I13453 BBa_B0034 BBa_K804002 BBa_B0025 | BBa_K804000 BBa_I13453 BBa_B0034 BBa_K804002 BBa_B0025 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K804000 BBa_I13453 BBa_B0034 BBa_K804002 BBa_B0025 | 1,921 930,1059 1068,1079 1086,1892 1901,2029 | BBa_K804000 BBa_I13453 BBa_B0034 BBa_K804002 BBa_B0025 |