BBa_K804009

BBa_K804009 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K804009

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Joe Ho

Date created: 2012-10-01 11:00:00

Date modified: 2015-05-08 01:13:25

Constitutive EYFP generator - Arabinose inducible TrpA coding gene

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K804009_sequence (Version 1)

Description

This part contains a constitutive EYFP generator along with an arabinose inducible (Pbad) Trp A coding gene. Upon induction with arabinose, TrpA is expressed for indole-3-glycerol-phosphate(InGP)binding and catalysis/cleavage of InGP to indole and glyceraldehyde-3-phosphate during an α reaction. This construct can be used to induce growth in Trp- auxotrophs and can be monitored by fluorescence for its population dynamics in co-culture with other auxotrophs.

Notes

None available.

Source

Strong constitutive promoter-EYFP generator-BBa_K804001
RBS-BBa_B0034
TrpA coding gene-BBa_K804002
Terminator- BBa_B0025

Components

Access	Instance	Definition
public	BBa_K804001	BBa_K804001
public	BBa_I13453	BBa_I13453
public	BBa_B0034	BBa_B0034
public	BBa_K804002	BBa_K804002
public	BBa_B0025	BBa_B0025

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K804001	1,921	BBa_K804001
BBa_I13453	930,1059	BBa_I13453
BBa_B0034	1068,1079	BBa_B0034
BBa_K804002	1086,1892	BBa_K804002
BBa_B0025	1901,2029	BBa_B0025

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K804009/1

Member of these Collections

• iGEM Parts Registry