Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Joe Ho
Date created: 2012-10-01 11:00:00
Date modified: 2015-05-08 01:13:25
Constitutive EYFP generator - Arabinose inducible MetA coding gene
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K804011_sequence (Version 1) |
Description
This part contains a constitutive EYFP generator along with an arabinose inducible (Pbad) Met A coding gene. Upon induction with arabinose, MetA is expressed for for the biosynthesis of de novo methionine. It encodes for the MetA enzyme which transfers a succinyl group from succinyl-CoA to homoserine, forming O-succinyl-L-homoserine, a precursor of methionine. This construct can be used to induce growth in Met- auxotrophs and can be monitored by fluorescence for its population dynamics in co-culture with other auxotrophs.Notes
None Available.Source
Strong constitutive promoter-EYFP generator- BBa_K804001Arabinose inducible MetA coding gene- BBa_K804010
| Access | Instance | Definition |
| public public | BBa_K804001 BBa_K804010 | BBa_K804001 BBa_K804010 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K804001 BBa_K804010 | 1,921 930,2152 | BBa_K804001 BBa_K804010 |