GEV

BBa_K807000 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_K807000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Ian Roney

Date created: 2012-09-27 11:00:00

Date modified: 2015-05-08 01:13:25

GEV - Gal4DNA Binding Domain-Human Estrogen Receptor - VP16 Activation Domain

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K807000_sequence (Version 1)

Description

This part is a synthetic transcriptional activator with specificity for the Gal4 upstream activating sequence that can be found in the Gal1 promoter of Saccharomyces cerevisiae. It is inactive in its native form but can be activated by the addition of Beta-Estradiol. The Beta-Estradiol acts to dimerize the activator.

Notes

Gal4 DBD and hER were designed without a stop codon so the full sequence would be a fusion protein.

Source

Gal4 DNA Binding Domain - Saccharomyces cerevisiae
Human Estrogen Receptor - Human
VP16 Activation Domain ??? Herpesvirus

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Gal4 DBD	1,278	feature/binding, non_covalent_binding_site
hER	279,1161	feature/protein, CDS
VP16	1162,1365	feature/tag, tag

Other Properties

• igem#experience: None
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K807000/1

Member of these Collections

• iGEM Parts Registry