Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Marie Burghard, Henrik Cordes, Jascha Diemer, Adrian Eilingsfeld, Sven Jager, Rene Sahm, Arne Wehling
Date created: 2012-09-01 11:00:00
Date modified: 2015-05-08 01:13:26
FsC: Cutinase PET cleaving enzyme
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K808025_sequence (Version 1) |
Description
A cutinase is a cuticula degrading hydrolase from the fungus Fusarium solani pisi. It shows activity towards PET.Notes
Surface Expression is very hard to do. FsC shows lipolytic activity and attacks the outer membrane of gramm negativ bacteria. This activity leads to a certain toxicity for expression hosts. In addition FsC needs to be expressed in the periplasm due to folding issues.Source
Cloned from vector pEST100 from Becker et all 2005:(Becker, S., Theile, S., Heppeler, N., Michalczyk, A., Wentzel, A., Wilhelm, S., Jaeger, K.-E., et al. (2005). A generic system for the Escherichia coli cell-surface display of lipolytic enzymes. FEBS letters, 579(5), 1177???82. doi:10.1016/j.febslet.2004.12.087)