Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Marie Burghard, Jascha Diemer, Adrian Eilingsfeld, Philipp Rottmann, Rene Sahm, Arne Wehling
Date created: 2012-09-01 11:00:00
Date modified: 2015-05-08 01:13:26
PhoA: signal sequence for peri plasmic expression
| Types | DnaRegion |
| Roles | Signalling engineered_region |
| Sequences | BBa_K808028_sequence (Version 1) |
Description
PhoA is an N-terminal signal sequence for expression into the peri plasma. It derives from the native phosphatase A of E.coli and is similar to pelB. We used it to express our fusion protein (BBa_K808030)Notes
As an signal peptide, our phoA has no stop codons and needs to be fused at the N-Terminus.Source
Our phoA BioBrick is derived from the pEST100 vector which was used in the following publication:Becker, S., Theile, S., Heppeler, N., Michalczyk, A., Wentzel, A., Wilhelm, S., Jaeger, K.-E., et al. (2005). A generic system for the Escherichia coli cell-surface display of lipolytic enzymes. FEBS letters, 579(5), 1177???82. doi:10.1016/j.febslet.2004.12.087