Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Cyrille Pauthenier
Date created: 2012-09-17 11:00:00
Date modified: 2015-05-08 01:13:27
GFP fused with AID-tag (ubuquitinase E3 OsTirI recoginition domain)
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K812010_sequence (Version 1) |
Description
This part is a super folded GFP fused to an AID tag for its recignition by the ubiquitinase E3 that induces its degradation in the presence of auxin in the eukaryote cell.This part is a part of a device pattented by Kanemaki Masato, Kakimoto Tatsuo, Nishimura Kohei, Takisawa Haruhiko and
Fukagawa Tatsuo for yeast and mammalian cells use (http://www.freepatentsonline.com/y2012/0115232.html). However the pattent does not cover the use for oviparian such as frogs and chicken.
The GFP-AID contains 2 NLS domains that relocate the protein in the nucleus of the cells for a better signal concentration.