BBa_K812031

BBa_K812031 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K812031
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chauris Karine
Date created: 2012-09-19 11:00:00
Date modified: 2015-05-08 01:13:27

sfGFP with kozak sequence for eukaryotic expression



    • Details

    Types
    DnaRegion

    Roles
    Reporter

    engineered_region

    Sequences BBa_K812031_sequence (Version 1)

    Description

    Super folded GFP fused with a kozak sequence to enhance its expression in eukaryotic cells. To be used with an eukaryotic expression system such as pSC2+ (K812000) and related parts.

    Notes

    PCR and cloned in pSB1C3 using EcoRI and PstI

    Source

    PCR amplified with kozak sequence from DNA coming from Alfonso Jaramillo's lab

    Sequence Annotation Location Component / Role(s)
    kozak
    sfGFP
    		1,6
    7,720
    		ribosome_entry_site feature/rbs
    CDS feature/protein

    igem#experience
    Works
     
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K812031/1
     

    Attachments

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