BBa_K812055

BBa_K812055 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K812055

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Cyrille Pauthenier

Date created: 2012-09-19 11:00:00

Date modified: 2015-05-08 01:13:27

Terminator B0015 with GoldenBrick extension

Details

• Types: DnaRegion
• Roles: Terminator; terminator
• Sequences: BBa_K812055_sequence (Version 1)

Description

B0015 terminator with GoldenBrick extension

This part is the GoldenBricked version of R0010 pLac promoter. The sequence is the same but the biobrick sequence have been modified to enable GoldenBrick assembly, adding a BsaI site and the possibility of BbsI digestion of the scar once formed.

To learn more about the GoldenBrick assembly our team have invented this year, go to: http://2012.igem.org/Team:Evry/GB

Notes

This part has been created from B0015 by PCR amplification

Source

The part B0015 has been amplified by PCR with the new GoldenBrick extension, and then cloned in pSB1C3 using EcoRI and PstI.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
GoldenBrick Terminator prefix	1,17	feature/BioBrick, engineered_region
EcoRI	1,6	feature/misc, sequence_feature
BsaI	7,12	feature/misc, sequence_feature
OH4	14,17	sequence_feature, feature/misc
XbaI	11,16	sequence_feature, feature/misc
BBa_B0010	18,97	stop_codon, feature/stop
BBa_B0012	106,146	feature/stop, stop_codon
GoldenBrick Terminator suffix	147,169	engineered_region, feature/BioBrick
SpeI	148,153	feature/misc, sequence_feature
OH5	154,157	feature/misc, sequence_feature
BsaI	159,164	feature/misc, sequence_feature
PstI	165,169	feature/misc, sequence_feature

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K812055/1

Member of these Collections

• iGEM Parts Registry